Coding

Part:BBa_M10035:Design

Designed by: Sadao Ota   Group: Berkeley BioE140L - S09   (2009-05-10)


{˂Ag43_long!} for Ag 43 autotransporter display system, including passenger domain


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 490
    Illegal PstI site found at 1114
    Illegal PstI site found at 1385
    Illegal PstI site found at 1438
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 490
    Illegal PstI site found at 1114
    Illegal PstI site found at 1385
    Illegal PstI site found at 1438
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 490
    Illegal PstI site found at 1114
    Illegal PstI site found at 1385
    Illegal PstI site found at 1438
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 490
    Illegal PstI site found at 1114
    Illegal PstI site found at 1385
    Illegal PstI site found at 1438
    Illegal NgoMIV site found at 382
    Illegal NgoMIV site found at 892
    Illegal NgoMIV site found at 913
    Illegal AgeI site found at 652
    Illegal AgeI site found at 1066
    Illegal AgeI site found at 1308
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The source of this part can be found from Escherichia coli str. K-12 substr. MG1655, complete genome. Due to the long length of the second part, you will not sequence your plasmid completely.


Source

Bglbricking of M10035, Ag43 (autotransporter and passenger) PCR Oso003 and Oso002 on E. coli MG1655 genomic DNA (1600 bp, EcoRI/BamHI) Sub into pBca9495KC-Bca1144#5 (EcoRI/BamHI, 910+2974, L) Product is pBca9495KC-M10035 {<Ag43_long!}

Oso003 Cloning of Ag43, long ccataGAATTCatgAGATCTacgctgacgctgaacgacag

Oso002 Cloning of Ag43 GCttaggatcctcagaaggtcacattcagtg

References