Coding
Part:BBa_M10035:Design
Designed by: Sadao Ota Group: Berkeley BioE140L - S09 (2009-05-10)
{˂Ag43_long!} for Ag 43 autotransporter display system, including passenger domain
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 490
Illegal PstI site found at 1114
Illegal PstI site found at 1385
Illegal PstI site found at 1438 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 490
Illegal PstI site found at 1114
Illegal PstI site found at 1385
Illegal PstI site found at 1438 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 490
Illegal PstI site found at 1114
Illegal PstI site found at 1385
Illegal PstI site found at 1438 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 490
Illegal PstI site found at 1114
Illegal PstI site found at 1385
Illegal PstI site found at 1438
Illegal NgoMIV site found at 382
Illegal NgoMIV site found at 892
Illegal NgoMIV site found at 913
Illegal AgeI site found at 652
Illegal AgeI site found at 1066
Illegal AgeI site found at 1308 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The source of this part can be found from Escherichia coli str. K-12 substr. MG1655, complete genome. Due to the long length of the second part, you will not sequence your plasmid completely.
Source
Bglbricking of M10035, Ag43 (autotransporter and passenger) PCR Oso003 and Oso002 on E. coli MG1655 genomic DNA (1600 bp, EcoRI/BamHI) Sub into pBca9495KC-Bca1144#5 (EcoRI/BamHI, 910+2974, L) Product is pBca9495KC-M10035 {<Ag43_long!}
Oso003 Cloning of Ag43, long ccataGAATTCatgAGATCTacgctgacgctgaacgacag
Oso002 Cloning of Ag43 GCttaggatcctcagaaggtcacattcagtg